Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV
Identifieur interne : 000853 ( Main/Exploration ); précédent : 000852; suivant : 000854Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV
Auteurs : Xiuyuan Ou [République populaire de Chine] ; Yan Liu [République populaire de Chine] ; Xiaobo Lei [République populaire de Chine] ; Pei Li [République populaire de Chine] ; Dan Mi [République populaire de Chine] ; Lili Ren [République populaire de Chine] ; Li Guo [République populaire de Chine] ; Ruixuan Guo [République populaire de Chine] ; Ting Chen [République populaire de Chine] ; Jiaxin Hu [République populaire de Chine] ; Zichun Xiang [République populaire de Chine] ; Zhixia Mu [République populaire de Chine] ; Xing Chen [République populaire de Chine] ; Jieyong Chen [République populaire de Chine] ; Keping Hu [République populaire de Chine] ; Qi Jin [République populaire de Chine] ; Jianwei Wang [République populaire de Chine] ; Zhaohui Qian [République populaire de Chine]Source :
- Nature Communications [ 2041-1723 ] ; 2020.
Descripteurs français
- KwdFr :
- Anticorps antiviraux (immunologie), Canaux calciques (métabolisme), Cathepsine L (métabolisme), Cathepsines (antagonistes et inhibiteurs), Cathepsines (métabolisme), Cellules HEK293, Cellules géantes (physiologie), Domaines protéiques, Endocytose, Fusion cellulaire, Glycoprotéine de spicule des coronavirus (), Glycoprotéine de spicule des coronavirus (immunologie), Glycoprotéine de spicule des coronavirus (métabolisme), Humains, Infections à coronavirus (immunologie), Multimérisation de protéines, Pandémies, Peptidyl-Dipeptidase A (métabolisme), Phosphatidylinositol 3-kinases (métabolisme), Pneumopathie virale (immunologie), Pénétration virale, Réactions croisées, Récepteurs viraux (métabolisme), Syndrome respiratoire aigu sévère (immunologie), Tests de neutralisation, Trypsine (métabolisme), Virus du SRAS (immunologie).
- MESH :
- antagonistes et inhibiteurs : Cathepsines.
- immunologie : Anticorps antiviraux, Glycoprotéine de spicule des coronavirus, Infections à coronavirus, Pneumopathie virale, Syndrome respiratoire aigu sévère, Virus du SRAS.
- métabolisme : Canaux calciques, Cathepsine L, Cathepsines, Glycoprotéine de spicule des coronavirus, Peptidyl-Dipeptidase A, Phosphatidylinositol 3-kinases, Récepteurs viraux, Trypsine.
- physiologie : Cellules géantes.
- Cellules HEK293, Domaines protéiques, Endocytose, Fusion cellulaire, Glycoprotéine de spicule des coronavirus, Humains, Multimérisation de protéines, Pandémies, Pénétration virale, Réactions croisées, Tests de neutralisation.
English descriptors
- KwdEn :
- Antibodies, Viral (immunology), Betacoronavirus (chemistry), Betacoronavirus (immunology), Betacoronavirus (physiology), Broadly Neutralizing Antibodies (immunology), Calcium Channels (metabolism), Cathepsin L (metabolism), Cathepsins (antagonists & inhibitors), Cathepsins (metabolism), Cell Fusion, Coronavirus Infections (immunology), Cross Reactions, Endocytosis, Giant Cells (physiology), HEK293 Cells, Humans, Neutralization Tests, Pandemics, Peptidyl-Dipeptidase A (metabolism), Phosphatidylinositol 3-Kinases (metabolism), Pneumonia, Viral (immunology), Protein Domains, Protein Multimerization, Receptors, Virus (metabolism), SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), Spike Glycoprotein, Coronavirus (chemistry), Spike Glycoprotein, Coronavirus (immunology), Spike Glycoprotein, Coronavirus (metabolism), Trypsin (metabolism), Virus Internalization.
- MESH :
- chemical , antagonists & inhibitors : Cathepsins.
- chemical , chemistry : Spike Glycoprotein, Coronavirus.
- chemical , immunology : Antibodies, Viral, Broadly Neutralizing Antibodies, Spike Glycoprotein, Coronavirus.
- chemistry : Betacoronavirus.
- immunology : Betacoronavirus, Coronavirus Infections, Pneumonia, Viral, SARS Virus, Severe Acute Respiratory Syndrome.
- chemical , metabolism : Calcium Channels, Cathepsin L, Cathepsins, Peptidyl-Dipeptidase A, Phosphatidylinositol 3-Kinases, Receptors, Virus, Spike Glycoprotein, Coronavirus, Trypsin.
- physiology : Betacoronavirus, Giant Cells.
- Cell Fusion, Cross Reactions, Endocytosis, HEK293 Cells, Humans, Neutralization Tests, Pandemics, Protein Domains, Protein Multimerization, Virus Internalization.
Abstract
Since 2002, beta coronaviruses (CoV) have caused three zoonotic outbreaks, SARS-CoV in 2002–2003, MERS-CoV in 2012, and the newly emerged SARS-CoV-2 in late 2019. However, little is currently known about the biology of SARS-CoV-2. Here, using SARS-CoV-2 S protein pseudovirus system, we confirm that human angiotensin converting enzyme 2 (hACE2) is the receptor for SARS-CoV-2, find that SARS-CoV-2 enters 293/hACE2 cells mainly through endocytosis, that PIKfyve, TPC2, and cathepsin L are critical for entry, and that SARS-CoV-2 S protein is less stable than SARS-CoV S. Polyclonal anti-SARS S1 antibodies T62 inhibit entry of SARS-CoV S but not SARS-CoV-2 S pseudovirions. Further studies using recovered SARS and COVID-19 patients’ sera show limited cross-neutralization, suggesting that recovery from one infection might not protect against the other. Our results present potential targets for development of drugs and vaccines for SARS-CoV-2.
Url:
DOI: 10.1038/s41467-020-15562-9
PubMed: 32221306
PubMed Central: 7100515
Affiliations:
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Le document en format XML
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type="main">Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV</title><author><name sortKey="Ou, Xiuyuan" sort="Ou, Xiuyuan" uniqKey="Ou X" first="Xiuyuan" last="Ou">Xiuyuan Ou</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0662 3178</institution-id><institution-id institution-id-type="GRID">grid.12527.33</institution-id><institution>NHC Key laboratory of Systems Biology of Pathogens,</institution><institution>Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College,</institution></institution-wrap>100176 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>100176 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Liu, Yan" sort="Liu, Yan" uniqKey="Liu Y" first="Yan" last="Liu">Yan Liu</name><affiliation wicri:level="1"><nlm:aff 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Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Mi, Dan" sort="Mi, Dan" uniqKey="Mi D" first="Dan" last="Mi">Dan Mi</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0662 3178</institution-id><institution-id institution-id-type="GRID">grid.12527.33</institution-id><institution>NHC Key laboratory of Systems Biology of Pathogens,</institution><institution>Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College,</institution></institution-wrap>100176 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>100176 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Ren, Lili" sort="Ren, Lili" uniqKey="Ren L" first="Lili" last="Ren">Lili Ren</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0662 3178</institution-id><institution-id institution-id-type="GRID">grid.12527.33</institution-id><institution>NHC Key laboratory of Systems Biology of Pathogens,</institution><institution>Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College,</institution></institution-wrap>100176 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>100176 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Guo, Li" sort="Guo, Li" uniqKey="Guo L" first="Li" last="Guo">Li Guo</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0662 3178</institution-id><institution-id institution-id-type="GRID">grid.12527.33</institution-id><institution>NHC Key laboratory of Systems Biology of Pathogens,</institution><institution>Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College,</institution></institution-wrap>100176 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>100176 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Guo, Ruixuan" sort="Guo, Ruixuan" uniqKey="Guo R" first="Ruixuan" last="Guo">Ruixuan Guo</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0662 3178</institution-id><institution-id institution-id-type="GRID">grid.12527.33</institution-id><institution>NHC Key laboratory of Systems Biology of Pathogens,</institution><institution>Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College,</institution></institution-wrap>100176 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>100176 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Chen, Ting" sort="Chen, Ting" uniqKey="Chen T" first="Ting" last="Chen">Ting Chen</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0662 3178</institution-id><institution-id institution-id-type="GRID">grid.12527.33</institution-id><institution>NHC Key laboratory of Systems Biology of Pathogens,</institution><institution>Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College,</institution></institution-wrap>100176 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>100176 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Hu, Jiaxin" sort="Hu, Jiaxin" uniqKey="Hu J" first="Jiaxin" last="Hu">Jiaxin Hu</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0662 3178</institution-id><institution-id institution-id-type="GRID">grid.12527.33</institution-id><institution>NHC Key laboratory of Systems Biology of Pathogens,</institution><institution>Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College,</institution></institution-wrap>100176 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>100176 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Xiang, Zichun" sort="Xiang, Zichun" uniqKey="Xiang Z" first="Zichun" last="Xiang">Zichun Xiang</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0662 3178</institution-id><institution-id institution-id-type="GRID">grid.12527.33</institution-id><institution>NHC Key laboratory of Systems Biology of Pathogens,</institution><institution>Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College,</institution></institution-wrap>100176 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>100176 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Mu, Zhixia" sort="Mu, Zhixia" uniqKey="Mu Z" first="Zhixia" last="Mu">Zhixia Mu</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0662 3178</institution-id><institution-id institution-id-type="GRID">grid.12527.33</institution-id><institution>NHC Key laboratory of Systems Biology of Pathogens,</institution><institution>Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College,</institution></institution-wrap>100176 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>100176 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Chen, Xing" sort="Chen, Xing" uniqKey="Chen X" first="Xing" last="Chen">Xing Chen</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0662 3178</institution-id><institution-id institution-id-type="GRID">grid.12527.33</institution-id><institution>Institute of Medicinal Plant Development (IMPLAD),</institution><institution>Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical Collage (PUMC),</institution></institution-wrap>151 Malianwa Road North, Haidian District, 100193 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>151 Malianwa Road North, Haidian District, 100193 Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Chen, Jieyong" sort="Chen, Jieyong" uniqKey="Chen J" first="Jieyong" last="Chen">Jieyong Chen</name><affiliation wicri:level="1"><nlm:aff id="Aff3">Hengshui Third People’s Hospital, Heibei, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hengshui Third People’s Hospital, Heibei</wicri:regionArea><wicri:noRegion>Heibei</wicri:noRegion></affiliation></author><author><name sortKey="Hu, Keping" sort="Hu, Keping" uniqKey="Hu K" first="Keping" last="Hu">Keping Hu</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0662 3178</institution-id><institution-id institution-id-type="GRID">grid.12527.33</institution-id><institution>Institute of Medicinal Plant Development (IMPLAD),</institution><institution>Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical Collage (PUMC),</institution></institution-wrap>151 Malianwa Road North, Haidian District, 100193 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>151 Malianwa Road North, Haidian District, 100193 Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Jin, Qi" sort="Jin, Qi" uniqKey="Jin Q" first="Qi" last="Jin">Qi Jin</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0662 3178</institution-id><institution-id institution-id-type="GRID">grid.12527.33</institution-id><institution>NHC Key laboratory of Systems Biology of Pathogens,</institution><institution>Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College,</institution></institution-wrap>100176 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>100176 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Jianwei" sort="Wang, Jianwei" uniqKey="Wang J" first="Jianwei" last="Wang">Jianwei Wang</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0662 3178</institution-id><institution-id institution-id-type="GRID">grid.12527.33</institution-id><institution>NHC Key laboratory of Systems Biology of Pathogens,</institution><institution>Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College,</institution></institution-wrap>100176 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>100176 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Qian, Zhaohui" sort="Qian, Zhaohui" uniqKey="Qian Z" first="Zhaohui" last="Qian">Zhaohui Qian</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0662 3178</institution-id><institution-id institution-id-type="GRID">grid.12527.33</institution-id><institution>NHC Key laboratory of Systems Biology of Pathogens,</institution><institution>Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College,</institution></institution-wrap>100176 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>100176 Beijing</wicri:regionArea></affiliation></author></analytic><series><title level="j">Nature Communications</title><idno type="eISSN">2041-1723</idno><imprint><date when="2020">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Viral (immunology)</term><term>Betacoronavirus (chemistry)</term><term>Betacoronavirus (immunology)</term><term>Betacoronavirus (physiology)</term><term>Broadly Neutralizing Antibodies (immunology)</term><term>Calcium Channels (metabolism)</term><term>Cathepsin L (metabolism)</term><term>Cathepsins (antagonists & inhibitors)</term><term>Cathepsins (metabolism)</term><term>Cell Fusion</term><term>Coronavirus Infections (immunology)</term><term>Cross Reactions</term><term>Endocytosis</term><term>Giant Cells (physiology)</term><term>HEK293 Cells</term><term>Humans</term><term>Neutralization Tests</term><term>Pandemics</term><term>Peptidyl-Dipeptidase A (metabolism)</term><term>Phosphatidylinositol 3-Kinases (metabolism)</term><term>Pneumonia, Viral (immunology)</term><term>Protein Domains</term><term>Protein Multimerization</term><term>Receptors, Virus (metabolism)</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Spike Glycoprotein, Coronavirus (chemistry)</term><term>Spike Glycoprotein, Coronavirus (immunology)</term><term>Spike Glycoprotein, Coronavirus (metabolism)</term><term>Trypsin (metabolism)</term><term>Virus Internalization</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps antiviraux (immunologie)</term><term>Canaux calciques (métabolisme)</term><term>Cathepsine L (métabolisme)</term><term>Cathepsines (antagonistes et inhibiteurs)</term><term>Cathepsines (métabolisme)</term><term>Cellules HEK293</term><term>Cellules géantes (physiologie)</term><term>Domaines protéiques</term><term>Endocytose</term><term>Fusion cellulaire</term><term>Glycoprotéine de spicule des coronavirus ()</term><term>Glycoprotéine de spicule des coronavirus (immunologie)</term><term>Glycoprotéine de spicule des coronavirus (métabolisme)</term><term>Humains</term><term>Infections à coronavirus (immunologie)</term><term>Multimérisation de protéines</term><term>Pandémies</term><term>Peptidyl-Dipeptidase A (métabolisme)</term><term>Phosphatidylinositol 3-kinases (métabolisme)</term><term>Pneumopathie virale (immunologie)</term><term>Pénétration virale</term><term>Réactions croisées</term><term>Récepteurs viraux (métabolisme)</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Tests de neutralisation</term><term>Trypsine (métabolisme)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Cathepsins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term><term>Broadly Neutralizing Antibodies</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Cathepsines</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Betacoronavirus</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Glycoprotéine de spicule des coronavirus</term><term>Infections à coronavirus</term><term>Pneumopathie virale</term><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Betacoronavirus</term><term>Coronavirus Infections</term><term>Pneumonia, Viral</term><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Calcium Channels</term><term>Cathepsin L</term><term>Cathepsins</term><term>Peptidyl-Dipeptidase A</term><term>Phosphatidylinositol 3-Kinases</term><term>Receptors, Virus</term><term>Spike Glycoprotein, Coronavirus</term><term>Trypsin</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Canaux calciques</term><term>Cathepsine L</term><term>Cathepsines</term><term>Glycoprotéine de spicule des coronavirus</term><term>Peptidyl-Dipeptidase A</term><term>Phosphatidylinositol 3-kinases</term><term>Récepteurs viraux</term><term>Trypsine</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Cellules géantes</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Betacoronavirus</term><term>Giant Cells</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Fusion</term><term>Cross Reactions</term><term>Endocytosis</term><term>HEK293 Cells</term><term>Humans</term><term>Neutralization Tests</term><term>Pandemics</term><term>Protein Domains</term><term>Protein Multimerization</term><term>Virus Internalization</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cellules HEK293</term><term>Domaines protéiques</term><term>Endocytose</term><term>Fusion cellulaire</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Multimérisation de protéines</term><term>Pandémies</term><term>Pénétration virale</term><term>Réactions croisées</term><term>Tests de neutralisation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="Par1">Since 2002, beta coronaviruses (CoV) have caused three zoonotic outbreaks, SARS-CoV in 2002–2003, MERS-CoV in 2012, and the newly emerged SARS-CoV-2 in late 2019. However, little is currently known about the biology of SARS-CoV-2. Here, using SARS-CoV-2 S protein pseudovirus system, we confirm that human angiotensin converting enzyme 2 (hACE2) is the receptor for SARS-CoV-2, find that SARS-CoV-2 enters 293/hACE2 cells mainly through endocytosis, that PIKfyve, TPC2, and cathepsin L are critical for entry, and that SARS-CoV-2 S protein is less stable than SARS-CoV S. Polyclonal anti-SARS S1 antibodies T62 inhibit entry of SARS-CoV S but not SARS-CoV-2 S pseudovirions. Further studies using recovered SARS and COVID-19 patients’ sera show limited cross-neutralization, suggesting that recovery from one infection might not protect against the other. 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Tsiodras</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Park, Yj" uniqKey="Park Y">YJ Park</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hulswit, Rjg" uniqKey="Hulswit R">RJG Hulswit</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huang, X" uniqKey="Huang X">X Huang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Simmons, G" uniqKey="Simmons G">G Simmons</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Gunaratne, Gs" uniqKey="Gunaratne G">GS Gunaratne</name></author><author><name sortKey="Yang, Y" uniqKey="Yang Y">Y Yang</name></author><author><name sortKey="Li, F" uniqKey="Li F">F Li</name></author><author><name sortKey="Walseth, Tf" uniqKey="Walseth T">TF Walseth</name></author><author><name sortKey="Marchant, Js" uniqKey="Marchant J">JS Marchant</name></author></analytic></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Ou, Xiuyuan" sort="Ou, Xiuyuan" uniqKey="Ou X" first="Xiuyuan" last="Ou">Xiuyuan Ou</name></noRegion><name sortKey="Chen, Jieyong" sort="Chen, Jieyong" uniqKey="Chen J" first="Jieyong" last="Chen">Jieyong Chen</name><name sortKey="Chen, Ting" sort="Chen, Ting" uniqKey="Chen T" first="Ting" last="Chen">Ting Chen</name><name sortKey="Chen, Xing" sort="Chen, Xing" uniqKey="Chen X" first="Xing" last="Chen">Xing Chen</name><name sortKey="Guo, Li" sort="Guo, Li" uniqKey="Guo L" first="Li" last="Guo">Li Guo</name><name sortKey="Guo, Ruixuan" sort="Guo, Ruixuan" uniqKey="Guo R" first="Ruixuan" last="Guo">Ruixuan Guo</name><name sortKey="Hu, Jiaxin" sort="Hu, Jiaxin" uniqKey="Hu J" first="Jiaxin" last="Hu">Jiaxin Hu</name><name sortKey="Hu, Keping" sort="Hu, Keping" uniqKey="Hu K" first="Keping" last="Hu">Keping Hu</name><name sortKey="Jin, Qi" sort="Jin, Qi" uniqKey="Jin Q" first="Qi" last="Jin">Qi Jin</name><name sortKey="Lei, Xiaobo" sort="Lei, Xiaobo" uniqKey="Lei X" first="Xiaobo" last="Lei">Xiaobo Lei</name><name sortKey="Li, Pei" sort="Li, Pei" uniqKey="Li P" first="Pei" last="Li">Pei Li</name><name sortKey="Liu, Yan" sort="Liu, Yan" uniqKey="Liu Y" first="Yan" last="Liu">Yan Liu</name><name sortKey="Mi, Dan" sort="Mi, Dan" uniqKey="Mi D" first="Dan" last="Mi">Dan Mi</name><name sortKey="Mu, Zhixia" sort="Mu, Zhixia" uniqKey="Mu Z" first="Zhixia" last="Mu">Zhixia Mu</name><name sortKey="Qian, Zhaohui" sort="Qian, Zhaohui" uniqKey="Qian Z" first="Zhaohui" last="Qian">Zhaohui Qian</name><name sortKey="Ren, Lili" sort="Ren, Lili" uniqKey="Ren L" first="Lili" last="Ren">Lili Ren</name><name sortKey="Wang, Jianwei" sort="Wang, Jianwei" uniqKey="Wang J" first="Jianwei" last="Wang">Jianwei Wang</name><name sortKey="Xiang, Zichun" sort="Xiang, Zichun" uniqKey="Xiang Z" first="Zichun" last="Xiang">Zichun Xiang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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